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[Stem cell separation with different cell separators].

R Eckstein1, V Weisbach, T Zeiler

  • 1Abteilung Innere Medizin und Poliklinik m.S. Hämatologie und Onkologie, Untersitätsklinikum Rudolf Virchow, Berlin.

Beitrage Zur Infusionstherapie = Contributions to Infusion Therapy
|January 1, 1991
PubMed
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Peripheral blood stem cell (PBSC) transplantation is increasingly successful. Continuous flow cell separators appear more effective than discontinuous flow machines for harvesting PBSC for autologous transplantation.

Area of Science:

  • Hematology
  • Oncology
  • Transplantation Medicine

Background:

  • Peripheral blood stem cell (PBSC) transplantation is gaining prominence over autologous bone marrow transplantation for various malignancies.
  • PBSC collection is a critical step in autologous transplantation procedures.

Purpose of the Study:

  • To compare the efficacy of different separation techniques and cell separators for harvesting PBSC.
  • To identify optimal methods for PBSC collection for autologous transplantation.

Main Methods:

  • Comparison of four distinct separation techniques.
  • Evaluation of three cell separators: COBE Spectra, Fresenius AS 104, and Haemonetics V50.
  • Assessment of cell harvesting efficiency in the context of autologous transplantation.

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Main Results:

  • Continuous flow cell separators demonstrated an advantage in harvesting PBSC compared to discontinuous flow machines.
  • Specific performance differences were observed among the evaluated cell separation devices.

Conclusions:

  • Continuous flow technology may offer superior outcomes for PBSC harvesting.
  • The choice of cell separator can impact the efficiency of PBSC collection for transplantation.