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Interferon enhances fibronectin expression in various cell types.

R K Maheshwari1, V P Kedar, D Bhartiya

  • 1Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland.

Journal of Biological Regulators and Homeostatic Agents
|July 1, 1990
PubMed
Summary
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Interferon (IFN) enhances fibronectin (FN) production and release by various cells. This study found that IFN increases both fibronectin synthesis and its messenger RNA levels in vitro.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Fibronectin (FN) is a crucial glycoprotein in wound healing, synthesized by various cells or from plasma.
  • Understanding factors influencing FN synthesis is vital for wound healing research.

Purpose of the Study:

  • To investigate the in vitro effect of interferon (IFN) on fibronectin (FN) synthesis and release.
  • To examine the impact of IFN on FN production across different cell types.

Main Methods:

  • Indirect immunofluorescence and immunoelectron microscopy were used to assess FN levels.
  • Metabolic labeling (35S-methionine), immunoprecipitation, and SDS-PAGE analyzed FN synthesis and release.
  • Poly (A)+ RNA isolation and cDNA probing determined FN mRNA levels in A549 cells.

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Main Results:

  • IFN treatment significantly increased FN staining in cells compared to controls.
  • Cells treated with IFN showed elevated FN synthesis and release into the culture medium.
  • IFN treatment led to increased fibronectin mRNA levels in human lung epithelial cells.

Conclusions:

  • Interferon (IFN) stimulates fibronectin (FN) synthesis and release in vitro.
  • IFN's effect on FN production occurs at both the protein and mRNA levels.
  • These findings suggest IFN may modulate wound healing processes through fibronectin.