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An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats
Published on: May 8, 2020
Myosin specific-T lymphocytes mediated myocardial inflammation in adoptive transferred rats
Jin Zhang1, Yuhua Liao, Xiang Cheng
1Laboratory of Cardiovascular Immunology, Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.
Abstract:
Myosin specific-T lymphocytes might mediate myocardial inflammation and remodeling after AMI. Myosin-activated or unactivated T lymphocytes in vitro were transferred into naive syngeneic rats, respectively. T lymphocyte infiltration and myocyte apoptosis were explored by the HE and TUNNEL. Proteins and mRNA levels of cytokines (IL-1beta, IL-6 and TNF-alpha) in myocardium were determined by RT-PCR and immunohisto-chemistry. T lymphocyte infiltration was evidently observed after one week of activated T cell transfer. The expressions of cytokines were elevated markedly one week later. The myocyte apoptosis occurred after T lymphocyte infiltration in myocardium. Our findings suggest that cardiac myosin activated-T lymphocytes may mediate myocardial inflammation and remodeling.
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