Identification of a necessary element for Toxoplasma gondii SAG1 gene expression

SAG1 codes for the stage-specific major surface antigen P30 of Toxoplasma gondii (T. gondii) tachyzoites. Six tandemly repeated, conserved 27 bp cassettes in the region from -231 to -70 bp were previously confirmed to be essential for high-level expression of SAG1 and serve as a positioning element directing the initiation of transcription. We demonstrate here that an element located between +19 and +28 bp is necessary for SAG1 gene expression by using deletion mutagenesis analysis and electrophoresis mobility shift assay (EMSA). This will provide an insight into the regulatory mechanisms of SAG1 gene expression.