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Related Experiment Videos

Lymphocyte E rosette inhibitory factor: a regulatory serum lipoprotein.

F V Chisari, T S Edgington

    The Journal of Experimental Medicine
    |November 1, 1975
    PubMed
    Summary

    Rosette inhibitory factor (RIF), a low-density lipoprotein (LDL) species, was isolated from hepatitis B virus-infected patients. RIF inhibits T lymphocyte E rosette function and mixed lymphocyte reactions, suggesting a role in viral-induced liver injury.

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    Area of Science:

    • Immunology
    • Biochemistry
    • Hepatology

    Background:

    • Rosette inhibitory factor (RIF) was previously identified in serum from hepatitis B virus (HBV)-infected patients.
    • RIF's precise nature and function remained largely undefined.

    Purpose of the Study:

    • To isolate and characterize RIF.
    • To investigate RIF's effects on lymphocyte function.
    • To explore RIF's potential role in HBV-associated liver injury.

    Main Methods:

    • Isolation and purification of RIF from patient serum.
    • Biochemical analysis of RIF composition, including apolipoproteins.
    • In vitro assays measuring RIF's effects on T lymphocyte E rosette function and mixed lymphocyte reactions.

    Main Results:

    • RIF identified as a minor species of beta-lipoprotein within the low-density lipoprotein (LDL) class, unrelated to HBV.
    • RIF is a complex macromolecule containing apolipoproteins A, B, and C.
    • RIF inhibits T lymphocyte E rosette function with high affinity and potency (1 x 10(-12) M).
    • RIF inhibits lymphocyte response to allogeneic cells in mixed lymphocyte reactions.
    • Lipoprotein fractions from normal serum showed similar inhibitory effects in mixed lymphocyte reactions.

    Conclusions:

    • RIF is a unique, functionally specific LDL species, potentially a lipoprotein complex or hybrid molecule.
    • RIF's association with HBV infection highlights the need for further investigation into its structure and role in viral-induced hepatocellular injury.

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