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Published on: May 21, 2020
[Analysis of the difference in proteome expression between yeast form and mould form of Penicillium marneffei using
Dong-Hua Liu1, Xiao-Jun Liu, Sheng-Shun Tan
1Derparment of Dermatology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. ldhys@sohu.com
Objective:
To compare the difference in protome expression between yeast form and mould form of Penicillium marneffei.
Methods:
Surface enhanced laser desorption/ionization (SELDI) time-of-flight mass spectra were performed to compare the expressed proteins between yeast form and mould form of Penicillium marneffei. Protein profiling was read by PBSIIC ProteinChip Reader and the proteome database was analyzed by Proteinchip Software 3.2.0.
Results:
Seventy-five distinct proteins were found in the yeast form and mould form of Penicillium marneffei, in which 10 proteins were up-regulated in yeast form and 3 in mould form. The proteins 2900 and 3151 were only expressed in the yeast form and the proteins 13,151 and 13,285 only in mould form.
Conclusion:
SELDI technique can identify the difference of the expressed low-molecular-mass proteins between the mould form and yeast form of Penicillium marneffei.
Insights
Surface-enhanced laser desorption/ionization (SELDI) identified distinct proteome expression differences between yeast and mould forms of Penicillium marneffei, revealing specific protein up-regulations in each form.
Area of Science:
- Mycology
- Proteomics
- Biochemistry
Context:
- Penicillium marneffei exhibits dimorphism, existing as a yeast form in hosts and a mould form in the environment.
- Understanding the proteomic differences between these forms is crucial for diagnosing and treating infections.
Purpose:
- To compare the proteome expression profiles of the yeast and mould forms of Penicillium marneffei.
- To identify differentially expressed proteins between the two morphological forms.
Summary:
- Surface-enhanced laser desorption/ionization (SELDI) time-of-flight mass spectrometry was employed to analyze protein expression in yeast and mould forms of Penicillium marneffei.
- Seventy-five distinct proteins were identified, with 10 up-regulated in the yeast form and 3 in the mould form. Specific proteins were exclusively detected in either the yeast or mould form.
Impact:
- SELDI technology effectively differentiates low-molecular-mass proteins between Penicillium marneffei's yeast and mould forms.
- This finding aids in understanding fungal dimorphism and can inform diagnostic strategies for Penicillium marneffei infections.
