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Related Experiment Videos

Turbo-mixing in microplates.

Elisa Mitre1, Margarete Schulze, Gerhard A Cumme

  • 1Institute of Biochemistry, Medical Faculty, Friedrich Schiller University Jena, Jena, Germany.

Journal of Biomolecular Screening
|January 30, 2007
PubMed
Summary
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Mixing small liquid volumes in microplates is now easier. A novel method uses an organic solvent drop to create turbulence, ensuring rapid and effective mixing without altering bioactive molecules.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Effective mixing of small liquid volumes in microplates is crucial for biochemical assays.
  • Current mixing methods can be time-consuming or alter sensitive biomolecules.

Purpose of the Study:

  • To introduce a novel, rapid, and non-disruptive method for mixing micro-volumes in microplates.
  • To evaluate the efficacy of organic solvent spotting for inducing turbulent mixing.

Main Methods:

  • Spotting small drops (1-3 microL) of organic solvents onto aqueous layers in microplate wells.
  • Monitoring mixing optically using pH-dependent dyes.
  • Assessing enzyme activity (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase) after mixing.

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Main Results:

  • Rapid mixing was achieved when the surface tension difference between the solvent drop and the bulk liquid exceeded 30 dynes/cm.
  • Factors like spotting parameters, viscosity, and well geometry influenced turbulence.
  • No significant difference in enzyme activity was observed compared to traditional shaking methods.

Conclusions:

  • Organic solvent spotting is an effective method for rapid micro-volume mixing in microplates.
  • This technique is compatible with enzyme assays and preserves molecular integrity.
  • The method offers a valuable alternative for microplate-based assays requiring efficient mixing.