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Artificial reporter gene providing MRI contrast based on proton exchange
Assaf A Gilad1, Michael T McMahon, Piotr Walczak
1The Russell H. Morgan Department of Radiology and Radiological Science, Neurosection, Division of MR Research, Traylor Building Rm. 217, 720 Rutland Ave., Baltimore, Maryland 21205, USA.
Abstract:
Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water relaxation to gain contrast. We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. This endogenous contrast, based on transfer of radiofrequency labeling from the reporter's amide protons to water protons, can be switched on and off.
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