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Updated: Jul 17, 2026
13:42
Quantitation of Rabies Virus in Various Bovine Brain Structures
Published on: May 22, 2021
Detection of rabies virus genes by in-situ polymerase chain reaction
P Ezhil Praveena1, R Jayakumar, C Balachandran
1Indian Veterinary Research Institute, Izatnagar, Bareilly.
Veterinary Research Communications
|January 30, 2007
Abstract:
Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the 'N' gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas.
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01:28Rabies
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02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...