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Does human attractin have DP4 activity?

Daniel Friedrich1, Torsten Hoffmann, Joachim Bär

  • 1Probiodrug AG, Weinbergweg 22, D-06120 Halle, Germany.

Biological Chemistry
|January 31, 2007
PubMed
Summary
This summary is machine-generated.

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Attractin, a protein linked to various conditions, was previously thought to be a protease. Research shows attractin is not a protease but functions as a receptor or adhesion protein.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Mutations in the attractin (ATRN) gene are associated with pigmentation, metabolism, immune status, and neurodegeneration.
  • The precise mechanisms of attractin's function remain unclear.
  • Attractin was initially identified as a circulating serum protein with postulated protease activity, similar to dipeptidyl peptidase 4 (DP4/CD26).

Purpose of the Study:

  • To investigate the enzymatic activity of human serum attractin.
  • To clarify the controversial role of attractin and DP4/CD26 in serum DP4 activity.
  • To determine whether attractin possesses protease activity or functions as a receptor/adhesion protein.

Main Methods:

  • Purification of attractin from human plasma.

Related Experiment Videos

  • Kinetic analysis and native electrophoresis of purified attractin.
  • Western blotting and N-terminal sequencing.
  • Adenosine deaminase (ADA) affinity chromatography to remove impurities.
  • Expression of different attractin isoforms.

    • Main Results:

    • Purified attractin exhibited DP4-like activity with kinetics similar to recombinant human DP4.
    • However, the electrophoretic behavior of this activity differed from known DP4 enzymes and matched attractin.
    • DP4 activity was traced to an impurity removable by ADA affinity chromatography, yielding pure attractin without DP4 activity.
    • Expressed attractin isoforms also lacked DP4 activity.

    Conclusions:

    • The observed DP4-like activity in purified plasma attractin was due to a DP4 enzyme impurity, not attractin itself.
    • Attractin does not possess DP4 protease activity.
    • The multidomain protein attractin likely functions as a receptor or adhesion molecule, not a protease.