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Updated: Jul 17, 2026
12:26
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
CDK13/CDC2L5 interacts with L-type cyclins and regulates alternative splicing
Hung-Hsi Chen1, Yu-Hui Wong, Anne-Marie Geneviere
1Institute of Neuroscience, National Yang-Ming University, Taipei 11221, Taiwan, ROC.
Biochemical and Biophysical Research Communications
|January 31, 2007
Summary
Cyclin-dependent kinase 13 (CDK13), formerly CDC2L5, interacts with L-type cyclins and regulates alternative splicing. This discovery strengthens the role of CDK12 and CDK13 as a kinase subfamily controlling gene expression through splicing modulation.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- CDC2L5 and CDK12 share sequence homology, suggesting a subfamily of CDC2-related kinases.
- CDK12 is known to interact with L-type cyclins and regulate alternative splicing.
- Previous research has identified PITAI/VRE motifs in this kinase subfamily.
Purpose of the Study:
- To investigate the interaction of CDC2L5 with L-type cyclins.
- To rename CDC2L5 to cyclin-dependent kinase 13 (CDK13) based on its function.
- To characterize the role of CDK13 in alternative splicing regulation.
Main Methods:
- Sequence homology analysis.
- Co-immunoprecipitation assays to study protein interactions.
- Subcellular localization studies.
- Overexpression of CDK13 and reporter constructs in HEK293T cells.
- Analysis of alternative splicing patterns of E1a transcripts.
Main Results:
- CDC2L5 was confirmed to interact with L-type cyclins, leading to its renaming as CDK13.
- The kinase domain of CDK13 is sufficient for binding L-type cyclins.
- CDK13 and L-type cyclins influence each other's subcellular localization.
- CDK13 alters E1a pre-mRNA splicing patterns in a dose-dependent manner.
- SF2/ASF and SC35 counteract the splicing effects of CDK13, similar to CDK12.
Conclusions:
- CDK13 is a novel regulator of alternative splicing.
- CDK12 and CDK13 form a subfamily of cyclin-dependent kinases involved in alternative splicing.
- These findings highlight the importance of CDK13 in post-transcriptional gene regulation.
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