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Removal of 3' nontranslated cDNA sequence improves interferon gamma synthesis in Escherichia coli
1Fraunhofer Institute for Toxicology and Molecular Biology, Hannover, Fed. Rep. of Germany.
Arzneimittel-Forschung
|December 1, 1991
Abstract:
The cDNA coding for human interferon gamma contains approximately 600 basepairs not translated sequence with 4 adenine/thymidine (AT)-rich clusters at its 3'end. The influence of this sequence on the expression of recombinant human Interferon gamma in Escherichia coli was investigated. Removal of the non-translated sequence and especially of the AT-rich clusters yielded an increase in expression of interferon gamma from 10 to 40% of total protein. These results may also be important for the production of other recombinant human proteins with similar 3' sequences in their cDNA.