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Preparation and characterization of monoclonal antibodies to substance P
P H Frickey1, J M Yanni, K Appell
1Clinical Products Research Laboratory, Eastman Kodak Company, Rochester, New York 14650-2117.
Hybridoma
|December 1, 1991
Abstract:
A series of mouse hybridomas that produced monoclonal antibodies reactive with Substance P was prepared in an attempt to find a surrogate NK-1 receptor. When the binding profile of one particular antibody, SubP14.36.1, was compared in a rank order plot with NK-1 receptors against various agonists and antagonists of Substance P, a high correlation was found between these two binding structures. In addition, this monoclonal antibody inhibited a functional assay for Substance P. This result indicated that the pharmacological effects of this peptide can be blocked by an antibody (Ab).