Related Experiment Videos
Molecular cloning and expression of the 01 rfb region from a pyelonephritic Escherichia coli 01:H1:K7
M J Ding1, C Svanborg, G E Haraguchi
1Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Microbial Pathogenesis
|November 1, 1991
Abstract:
The genes responsible for the biosynthesis of the O1 polysaccharide from a human pyelonephritic Escherichia coli were cloned and expressed in a rfb-deleted E. coli K-12 strain. Deletion analysis of the clone demonstrated that a DNA fragment size larger than 6.7 kb and smaller than 10 kb is responsible for O1-antigen biosynthesis.