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Embedded Bioprinting of Tissue-like Structures Using κ-Carrageenan Sub-Microgel Medium
Published on: May 3, 2024
Esophageal muscle cell interaction with biopolymers
Mevlit Korkmaz1, Tahsin Yakut, Adnan Narci
1Department of Pediatric Surgery, Afyon Kocatepe University Medical Faculty, Afyon, Turkey. mevlitkorkmaz@hotmail.com
Summary
Synthetic absorbable polymers like poly-lactic-co-glycolic acid (PLGA) effectively support esophageal smooth muscle cell (SMC) growth and tissue formation. PLGA scaffolds demonstrated superior muscle regeneration compared to polyglycolic acid (PGA).
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Investigating the interaction between esophageal smooth muscle cells (SMCs) and synthetic absorbable polymers.
- Evaluating artificial muscle tissues developed from subcutaneous implantation of cell-polymer constructs.
Purpose of the Study:
- To assess the efficacy of polyglycolic acid (PGA) and poly-lactic-co-glycolic acid (PLGA) scaffolds in supporting esophageal smooth muscle cell growth and tissue formation.
- To compare the performance of PGA and PLGA in promoting muscle tissue regeneration in vitro and in vivo.
Main Methods:
- Esophageal SMCs (adult and fetal rabbits) were cultured and identified via immunostaining.
- Cells were seeded onto PGA and PLGA scaffolds for 3-D culture and assessed for proliferation and cell-polymer interactions.
- Cell-seeded scaffolds were implanted subcutaneously in rabbits for four weeks and analyzed histologically for muscle formation.
Main Results:
- Esophageal SMCs expressed actin and myosin, confirming their identity.
- Intensive cell attachment to PGA and PLGA fibers was observed after 48 hours of cultivation.
- Both PGA and PLGA supported cell proliferation, with PLGA scaffolds showing enhanced muscle formation compared to PGA.
Conclusions:
- Scaffolds serve as crucial vehicles for cell delivery in tissue engineering.
- Thorough testing of material-cell interactions is essential prior to clinical application.
- A 3-D agar gel culture system provides a practical method for assessing material biocompatibility and toxicity.
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