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Related Experiment Video

Updated: Jul 17, 2026

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Published on: April 16, 2021

Microbiologyc control in human heart valves.

Angela Maria Peruzzo¹, Francisco Diniz Affonso da Costa, Wanda Moscalewski Abrahão

¹Cardioprótese--Ind. e Com. de Produtos para Saúde, Pontifícia Universidade Católica do Paraná, Universidade Federal do Paraná, Passo Fundo, RS, Brazil. angelamariaperuzzo@yahoo.com.br

Arquivos Brasileiros De Cardiologia

|January 31, 2007

Summary

Microbiological evaluation of 1,671 human heart valves found 92% suitable for cardiovascular surgery. Strict controls are vital to prevent contamination and ensure patient safety during valve processing.

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Last Updated: Jul 17, 2026

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

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Published on: April 16, 2021

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Published on: October 19, 2015

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Published on: January 7, 2019

Area of Science:

Cardiovascular Surgery
Microbiology
Biomaterials Science

Background:

Human heart valves are critical for cardiovascular surgeries.
Sterilization and microbiological safety are paramount for allograft viability.
The Human Heart Valve Bank of Santa Casa de Misericórdia of Curitiba processes valves for transplantation.

Purpose of the Study:

To assess the microbiological quality of processed human heart valves.
To determine the suitability of valves for cardiovascular surgery based on microbial contamination.
To evaluate the effectiveness of the valve processing methods used by the Human Heart Valve Bank.

Main Methods:

Microbiological analysis of 1,671 human heart valves processed between July 1999 and June 2004.
Samples of valves and processing solutions were cultured using modified Brazilian Pharmacopeia (1988) and USP (1990) methodologies.
Incubation for 14 days followed by microbial identification of any contaminants.

Main Results:

92% of the 1,671 processed valves met microbiological standards for clinical use.
8% of the valves were rejected due to microbial contamination identified during processing.
No significant issues were reported regarding the culture media or identification methods.

Conclusions:

Rigorous microbiological control is essential for human grafts.
Preventing the use of contaminated valves in cardiovascular surgery is crucial for patient outcomes.
The valve processing procedures at the Human Heart Valve Bank demonstrated a high degree of microbiological safety.