HPLC Analysis of the Main Furanocoumarins from Peucedanum palustre

The main furanocoumarins from the roots of PEUCEDANUM PALUSTRE were separated with a RP-HPLC method. The mobile phase for the separation of the eight main compounds (oxypeucedanin hydrate, bergapten, oxypeucedanin, isobyakangelicin angelate, ostruthol, imperatorin, isoimperatorin, and columbianadin) was optimized with the "PRISMA" model, which is a multi-solvent optimization system. The chromatographic method can be applied for quantitative determination, as was demonstrated for different samples of P. PALUSTRE. The purity and identity of the peaks were controlled by diode-array detection and comparison with standards.