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Updated: Jul 17, 2026

A Comprehensive Approach to Analyze the Cell Components of Cerebral Blood Clots
Published on: July 21, 2023
[A component of earthworm fibrinolytic enzyme having higher thrombolytic activity than total components in vivo]
Xiao-yu Zhao1, Ze-ming Liu, Tian-yu Jing
1Life Science College of Hebei University, Baoding 071002, China. uyoaix@yahoo.com.cn
Aim:
To select higher thrombolytic and lower toxic single component of earthworm fibrinolytic enzymes (EFE).
Methods:
EFE containing total components were obtained by affinity chromatography from Eisenia fetida. Using ion-exchange chromatography to separate three main components EfP-0-2, EfP-I-1 and EfP-I-2 from EFE, their thrombolytic activity and toxicity were compared with EFE.
Results:
Among these components, EfP-I-1 had higher thrombolytic activity in vitro. When 4.5 mg x kg(-1) of these components were injected, the contents of fibrinogen in rat serum were not affected, but only EfP-I-1 exhibited distinct thrombolytic activity. When 6.0 mg x kg(-1) of them were injected intravenously, the bleeding time was not evidently delayed only by EfP-I-1. The acute toxicity test showed that the LD50 of EfP-I-1 was higher than EFE by 2. 17 times.
Conclusion:
Because of distinct thrombolytic activity, lower toxicity in vivo, higher content in EFE and easy to purify, EfP-I-1 was adapted to be developed as a single component medicine for treating thrombus.
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