Assays for testing Pneumocystis carinii viability
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E S Kaneshiro1, Y P Wu, M T Cushion
1Department of Biological Sciences, University of Cincinnati, OH 45221-0006.
The Journal of Protozoology
|November 1, 1991
Abstract:
A series of classical vital stains and fluorescent indicator compounds were evaluated as viability assays of P. carinii. The combination of the acetoxymethyl ester of calcein with either ethidium homodimer or propidium iodide distinguished between live, dead and moribund organisms and provided high fluorescence intensity and low bleaching enabling photodocumentation at high magnifications.