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Updated: Jul 17, 2026

Light-driven Enzymatic Decarboxylation
Published on: May 22, 2016
Synthesis and characterization of 4-hydroxy-2-nonenal derivatives for gas chromatographic analysis with electron
Enzo Santaniello1, Alessandro Repetto, Luca Maria Chiesa
1Dipartimento di Medicina, Chirurgia e Odontoiatra, Facoltà di Medicina e Chirurgia, Università degli Studi di Milano, Milano, Italy. enzo.santaniello@unimi.it
Abstract:
4-Hydroxy-2-nonenal (HNE) has been prepared from the corresponding dimethylacetal (HNE-DMA), in turn synthesized by a conventional approach with a few modifications of the experimental protocol and some improvements in the purification of the final product. In order to exploit the sensitivity of gas-chromatography with electron capture detector (GC-ECD) in the analysis of HNE derivatives, reaction of HNE with 2,4,6-trichlophenylhydrazine (TCPH) and 3,5-dichloro-phenylhydrazine (DCPH) was tested. Reaction with TCPH afforded a mixture of products, whereas with DCPH a single major product was formed that was prepared on a millimolar scale and purified. (1)H-NMR analysis established that the derivative of HNE with DCPH is HNE 3,6-dichloro-phenylhydrazone, that can be used as standard for GC-ECD analysis.
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