Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
Acute Kidney Injury V: Interprofessional Care01:20

Acute Kidney Injury V: Interprofessional Care

Acute Kidney Injury (AKI) requires a collaborative healthcare approach to restore renal function and prevent complications. Essential management strategies involve monitoring fluid and electrolyte balance, adjusting medications, initiating dialysis when necessary, and providing nutritional support.Fluid and Electrolyte ManagementFluid Monitoring: Regularly monitoring body weight, central venous pressure, and urine output helps detect fluid imbalances early. Patient intake and output are...
Overview of Protein Metabolism01:21

Overview of Protein Metabolism

Proteins are broken down into amino acids during digestion. Unlike fats and carbohydrates, which are stored for later use, proteins are not. Instead, amino acids are either used to produce ATP through oxidation or contribute to the creation of new proteins for the growth and repair of the body. Any surplus amino acids from the diet are converted into glucose or triglycerides rather than excreted.
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Hemodialysis II: Procedure and Complications01:24

Hemodialysis II: Procedure and Complications

DialyzersA hemodialysis (HD) dialyzer is a plastic cartridge containing thousands of parallel hollow fibers, which serve as semipermeable membranes. These fibers are typically made from cellulose-based or other synthetic materials. During HD, blood is pumped into the top of the cartridge and distributed among these fibers. Simultaneously, dialysis fluid, known as dialysate, is introduced into the bottom of the cartridge, bathing the outside of the fibers. Across the semipermeable membrane,...
Hemodialysis III: Nursing Management01:25

Hemodialysis III: Nursing Management

The nursing management of a patient undergoing hemodialysis includes several critical steps, starting with a thorough assessment before the procedure.Before the Hemodialysis ProcedureFirst, record the patient's vital signs—blood pressure, heart rate, respiratory rate, and temperature—to establish a baseline. This baseline is essential for detecting conditions such as hypotension that could impact the patient's response to dialysis. Document the patient's pre-dialysis weight, as this measurement...
Amino Acid Catabolism01:18

Amino Acid Catabolism

Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Can one use serum Lactate Concentration to Correct for the Anion gap?

Journal of intensive care medicine·2026
Same author

Classifying Hyponatremias According to Tonicity Disorder: Hypotonic Hyponatremia, Hypertonic Hyponatremia, and Pseudohyponatremia as Distinct Entities.

Cureus·2025
Same author

A Comparison of Derivatives of Alanine and d-Alanine Used in Gas Chromatography-Mass Spectrometry Analysis for Protein Kinetics.

Journal of proteome research·2025
Same author

Concurrent Control of Sodium and Bicarbonate Serum Concentrations Using a Four-Stream Hemodialysis Fluid Delivery System.

Hemodialysis international. International Symposium on Home Hemodialysis·2025
Same author

Quantifying the Deficits of Body Water and Monovalent Cations in Hyperglycemic Emergencies.

Journal of clinical medicine·2025
Same author

Editorial: Dysnatremias and related disorders.

Frontiers in medicine·2024

Related Experiment Video

Updated: Jul 17, 2026

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
06:32

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Published on: April 13, 2022

Amino acid repletion does not decrease muscle protein catabolism during hemodialysis.

Dominic S C Raj1, Oladipo Adeniyi, Elizabeth A Dominic

  • 1Division of Nephrology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131-5271, USA. draj@salud.unm.edu

American Journal of Physiology. Endocrinology and Metabolism
|February 1, 2007
PubMed
Summary

Amino acid infusion during hemodialysis (HD) increased muscle protein synthesis but did not reduce muscle breakdown in end-stage renal disease (ESRD) patients. Muscle protein catabolism persisted despite increased amino acid supply.

More Related Videos

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin
07:38

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

Published on: May 6, 2018

Related Experiment Videos

Last Updated: Jul 17, 2026

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
06:32

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Published on: April 13, 2022

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin
07:38

Induction of Nephrotic Syndrome in Mice by Retrobulbar Injection of Doxorubicin and Prevention of Volume Retention by Sustained Release Aprotinin

Published on: May 6, 2018

Area of Science:

  • Nephrology
  • Metabolic Research
  • Muscle Physiology

Background:

  • Intradialytic protein catabolism in end-stage renal disease (ESRD) patients is linked to amino acid loss during hemodialysis (HD).
  • Understanding muscle protein turnover and amino acid kinetics during HD is crucial for nutritional management.

Purpose of the Study:

  • To investigate the impact of amino acid infusion during HD on muscle protein turnover and amino acid transport kinetics in ESRD patients.
  • To determine if amino acid repletion can mitigate muscle protein breakdown during hemodialysis.

Main Methods:

  • Stable isotope tracers (phenylalanine, leucine, lysine) were used to study eight ESRD patients.
  • Muscle protein turnover and amino acid transport were measured at baseline, during HD without amino acid infusion (HD-O), and during HD with amino acid infusion (HD+AA).

Main Results:

  • Amino acid depletion during HD-O increased outward amino acid transport from muscle.
  • HD+AA facilitated amino acid transport into muscle cells, but muscle protein breakdown exceeded synthesis.
  • Despite amino acid supply, net muscle protein balance remained negative, and protein synthesis efficiency decreased.

Conclusions:

  • Amino acid infusion during HD enhances muscle protein synthesis but does not reduce muscle protein breakdown in ESRD patients.
  • Muscle protein catabolism persists during hemodialysis even with amino acid repletion, suggesting complex regulatory mechanisms.