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Laser Capture Microdissection of Paraformaldehyde-Fixed Mouse Liver Tissue for RNA Analysis
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Direct polymerase chain reaction amplification of formalin-fixed, paraffin-wax-embedded tissue after automated

S L O'Kane1, V Garimella, N Sivarajasingham

  • 1Cancer Biology Proteomics Group, Postgraduate Medical Institute of the University of Hull in association with the Hull-York Medical School, University of Hull, Hull, UK.

Journal of Clinical Pathology
|February 1, 2007
PubMed

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...