Related Experiment Videos
Enzymatic fragment substitution as a tool in protein design
H Tschesche1, C Groeger, H R Wenzel
1Universität Bielefeld, Fakultät für Chemie, Lehrstuhl für Biochemie, Germany.
Biomedica Biochimica Acta
|January 1, 1991
Abstract:
An easy and rapid enzymatic method is described which allows replacement of P'-residues in bovine pancreatic trypsin inhibitor. Insertion of Xaa-Arg or Xaa-Lys into a BPTI fragment lacking P1' = Ala16 and P2' = Arg17 was carried out in a "one pot" reaction catalysed by trypsin in the presence of 80% 1,4 butanediol.