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Anti-phosphotyrosine antibodies--obtaining and purification
M Stefănescu1, L Cremer, C Cristescu
1Cantacuzino Institute, Bucharest, Romania.
Roumanian Archives of Microbiology and Immunology
|July 1, 1991
Abstract:
Anti-phosphotyrosine antibodies were obtained by rabbits immunization using bovine serum albumin-phosphotyrosine complex. Hyperimmune rabbit serum was purified on affinity chromatography column obtained by phosphotyramine binding on Sepharose 4 B, CNBr-activated. The antibodies obtained after purification were tested by ELISA and immunoblotting.