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Updated: Jul 26, 2026
Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Published on: September 14, 2013
Preparation of antibacterial and antitoxic Clostridium difficile sera
B Sokół-Leszczyńska1, G Martirossian, F Meisel-Mikołajczyk
1Department of Bacteriology and Immunology, Institute of Biostructure Medical Academy, Warsaw, Poland.
Abstract:
Preparation of Clostridium difficile antibacterial and antitoxic sera is presented. Fifty one strains (72%) were typeable within Delmee scheme. Twenty strains (28%) belonged to new Polish serogroups designated 18, 27, 70, 71, 72, 88, 89 and NICH. Supernatants of all toxigenic Clostridium difficile strains were neutralized by gamma-globulin fraction of goat Clostridium difficile antitoxin in neutralization assay when it was performed on McCoy cell line. Only 8 toxigenic strains (21%) were positive in counterimmunoelectrophoresis.
