Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of Prenatal Alcohol Exposure on Cerebral Cortex Development.

Advances in experimental medicine and biology·2026

Same author

[Reform of the law on compulsory admission in Belgium: more tailored healthcare?]

Tijdschrift voor psychiatrie·2024

Same author

Management of patients with a neurovascular conflict involving the optic nerve and a non-diseased intracranial artery: Three cases.

Brain & spine·2024

Same author

Food system actor perspectives on future-proofing European food systems through plant breeding.

Scientific reports·2023

Same author

Blood flow restriction training in South Africa - a panel discussion.

South African journal of sports medicine·2023

Same author

[Use of depot antipsychotics in Belgium between 1997 and 2016].

Tijdschrift voor psychiatrie·2021

Search research articles

Related Experiment Video

Updated: Jul 17, 2026

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

Gelatin-based microcarriers as embryonic stem cell delivery system in bone tissue engineering: an in-vitro study.

S Tielens¹, H Declercq, T Gorski

¹Department of Anatomy, Embryology, Histology, and Medical Physics, Ghent University, L. Pasteurlaan 2, B-9000 Ghent, Belgium.

Biomacromolecules

|February 3, 2007

Summary

Mouse embryonic stem cells were successfully cultured on microcarriers and differentiated into bone cells. Optimal cell viability was achieved using specific polymer encapsulation techniques without gelatin.

More Related Videos

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

Published on: April 21, 2021

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Published on: August 8, 2022

Related Experiment Videos

Last Updated: Jul 17, 2026

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

Published on: April 21, 2021

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs

Published on: August 8, 2022

Area of Science:

Biomaterials Science
Stem Cell Biology
Tissue Engineering

Background:

Biodegradable macroporous microcarriers offer a scaffold for cell culture.
Maintaining pluripotency and inducing differentiation are key for stem cell applications.
Encapsulation techniques are crucial for cell viability in biomaterial constructs.

Purpose of the Study:

To culture mouse embryonic stem cells on microcarriers.
To induce osteogenic differentiation of these cells.
To optimize encapsulation of cell-loaded microcarriers in photopolymerizable polymers for enhanced cell viability.

Main Methods:

Culture of mouse embryonic stem cells on macroporous microcarriers.
Induction of osteogenic differentiation using specific media.
Encapsulation of cell-laden microcarriers within methacrylate-endcapped poly-D,L-lactide-co-caprolactone using triacetin or HEMA as solvents, with or without gelatin.
Transmission electron microscopy for cell viability assessment.

Main Results:

Embryonic stem cells colonized microcarriers within 1-2 weeks.
Pluripotency was maintained for up to 14 days with leukemia inhibitory factor.
Osteogenic differentiation was initiated after 2 weeks in differentiation medium.
Homogeneous microcarrier distribution was achieved in the polymer matrix.
Optimal cell viability was observed when gelatin was omitted and triacetin was used as a solvent.

Conclusions:

Biodegradable macroporous microcarriers support mouse embryonic stem cell culture and osteogenic differentiation.
Photopolymerizable polymer encapsulation is feasible for cell-loaded microcarriers.
The choice of solvent and the exclusion of gelatin are critical for maximizing cell viability in the encapsulated constructs.