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Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
Published on: October 28, 2016
Large scale preparation of the mammalian high mobility group protein A2 for biophysical studies
Tengjiao Cui1, Suzanne Joynt, Victor Morillo
1Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199, USA.
Protein and Peptide Letters
|February 3, 2007
Abstract:
Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.
