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Detection and Isolation of Campylobacter spp. from Raw Meat
Published on: February 23, 2024
Isolation of a gene that is involved in Campylobacter jejuni 81116 cytotoxin activation
Kaiyan Liu1, Benjamin N Fry, Peter J Coloe
1Landguard Development Facility, Orica Australia Pty Ltd. Bundoora, Victoria, Australia.
Abstract:
Cytotoxin fractions were isolated from Campylobacter jejuni 81116 and semi-purified by size-exclusion liquid chromatography. The fraction showing the strongest toxicity was injected into mice to produce antiserum. The antiserum was used to screen a C. jejuni 81116 cosmid library. Nine genes were identified in overlapping cosmid inserts that induced reactivity with the antiserum. One of these genes showed high similarity to a periplasmic protein of unknown function and its isogenic mutant showed decreased toxicity compared to the C. jejuni 81116 wild type. This gene contains a Gram-negative bacterial RTX toxin-activating protein C signature, which suggests it may play a role in C. jejuni 81116 cytotoxin activation.
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