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Related Experiment Videos

Expression of terminal complement components by human keratinocytes.

Krisztina K Timár1, Attila Dallos, Mária Kiss

  • 1Department of Dermatology, Academic Medical Center, University of Amsterdam, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands. k.k.timar@amc.uva.nl

Molecular Immunology
|February 3, 2007
PubMed
Summary

Human keratinocytes synthesize terminal complement components C5, C7, C8gamma, and C9. Tumor necrosis factor-alpha (TNF-alpha) specifically upregulates C9 production in these skin cells.

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Area of Science:

  • Immunology
  • Dermatology
  • Complement System Biology

Background:

  • Human keratinocytes are key players in the skin's immune system, producing cytokines and complement proteins.
  • Previous studies identified keratinocyte synthesis of complement components C3, factor B, factor H, and factor I.
  • The synthesis and regulation of terminal complement components by keratinocytes remained largely uninvestigated.

Purpose of the Study:

  • To investigate the synthesis of terminal complement components (C5-C9) by human keratinocytes.
  • To examine the regulatory effects of specific cytokines (IL-1alpha, IL-2, IL-6, TGF-beta1, TNF-alpha, IFN-gamma) on terminal complement component synthesis.

Main Methods:

  • Analysis of mRNA expression for complement components C5-C9 in human keratinocytes.

Related Experiment Videos

  • Detection of secreted complement proteins C5-C9 in keratinocyte culture supernatants.
  • Treatment of keratinocytes with various cytokines to assess regulatory impacts on complement synthesis.

    • Main Results:

    • Human keratinocytes constitutively express mRNA for C5, C7, C8gamma, and C9, but not C6, C8alpha, or C8beta.
    • Keratinocytes secrete C7 and C9, but not C5, C6, or C8.
    • Only TNF-alpha significantly upregulated the production of C9 among the tested cytokines.

    Conclusions:

    • Human keratinocytes synthesize select terminal complement components, including C7 and C9.
    • The synthesis of C9 by keratinocytes is subject to regulation, notably by TNF-alpha.
    • These findings expand the understanding of keratinocytes' role in the complement system within the skin immune response.