Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cell-free translation system using phosphorothioate-containing mRNA.

T Ueda1, H Tohda, N Chikazumi

  • 1Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

Nucleic Acids Symposium Series
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of weight loss on the progression of meniscal extrusion and size in knee osteoarthritis: a post-hoc analysis of the Intensive Diet and Exercise for Arthritis (IDEA) trial.

Osteoarthritis and cartilage·2020
Same author

Radiographically normal knees with contralateral joint space narrowing display greater change in cartilage transverse relaxation time than those with normal contralateral knees: a model of early OA? - data from the Osteoarthritis Initiative (OAI).

Osteoarthritis and cartilage·2019
Same author

Cartilage loss in radiographically normal knees depends on radiographic status of the contralateral knee - data from the Osteoarthritis Initiative.

Osteoarthritis and cartilage·2018
Same author

Trajectories of femorotibial cartilage thickness among persons with or at risk of knee osteoarthritis: development of a prediction model to identify progressors.

Osteoarthritis and cartilage·2018
Same author

The role of thigh muscle and adipose tissue in knee osteoarthritis progression in women: data from the Osteoarthritis Initiative.

Osteoarthritis and cartilage·2018
Same author

Is local or central adiposity more strongly associated with incident knee osteoarthritis than the body mass index in men or women?

Osteoarthritis and cartilage·2018

Incorporating phosphorothioate into messenger RNA (mRNA) enhances protein synthesis efficiency by stabilizing the mRNA molecules in cell-free systems. This modification also proves effective in continuous cell-free protein production.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • RNA Therapeutics

Background:

  • Messenger RNA (mRNA) stability is crucial for efficient protein synthesis.
  • Existing cell-free translation systems face limitations in mRNA longevity and protein yield.
  • Phosphorothioate modification offers a potential strategy to enhance RNA stability.

Purpose of the Study:

  • To investigate the impact of phosphorothioate incorporation into RNA on protein synthesis efficiency.
  • To evaluate the stability of phosphorothioate-containing mRNAs in prokaryotic cell-free translation systems.
  • To assess the applicability of these modified mRNAs in continuous cell-free translation systems.

Main Methods:

  • Generation of phosphorothioate-containing RNAs via in vitro transcription using T7 RNA polymerase and ribonucleoside 5'-O-(1-thiotriphosphates) (NTP alpha S).

Related Experiment Videos

  • Assessment of protein synthesis efficiency using prokaryotic cell-free translation systems.
  • Evaluation of mRNA stability under cell-free conditions.
  • Testing the performance of modified mRNAs in a continuous cell-free translation system.

    • Main Results:

    • Phosphorothioate substitution significantly increased the efficiency of protein synthesis.
    • The modified mRNAs exhibited enhanced stability in prokaryotic cell-free translation systems.
    • The substituted mRNAs demonstrated successful application in a continuous cell-free translation system.

    Conclusions:

    • Phosphorothioate-containing RNAs are more stable and lead to increased protein synthesis efficiency in cell-free systems.
    • This RNA modification strategy holds promise for improving cell-free protein production technologies.
    • The findings support the use of phosphorothioate-modified mRNAs for enhanced biotechnological applications.