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Related Experiment Video

Updated: Jul 17, 2026

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles

Published on: April 8, 2012

Gene transfer in human skin with different pseudotyped HIV-based vectors.

A Hachiya¹, P Sriwiriyanont, A Patel

¹Kao Biological Science Laboratories, Haga, Tochigi, Japan. hachiya.akira@kao.co.jp

|February 3, 2007

Summary

This study explored using pseudotyped lentiviral vectors for genetic skin treatments. Vesicular stomatitis virus glycoprotein (VSV-G) showed the most efficient gene delivery to human skin stem cells.

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Area of Science:

Gene therapy
Virology
Dermatology

Background:

Lentiviral vectors pseudotyped with viral envelope glycoproteins can modify tropism for gene delivery.
Targeting human skin with gene therapy holds potential for treating genetic anomalies.

Purpose of the Study:

To evaluate the efficiency of different pseudotyped human immunodeficiency virus (HIV)-based vectors for gene transfer in human skin xenografts.
To identify the most effective viral glycoprotein for cutaneous gene delivery.

Main Methods:

Pseudotyping HIV vectors with envelope glycoproteins from vesicular stomatitis virus (VSV-G), Zaire Ebola (EboZ) virus, murine leukemia virus (MuLV), lymphocytic choriomeningitis virus (LCMV), Rabies, or Mokola virus.
Qualitative and quantitative assessment of transgene expression (LacZ reporter gene) in human skin xenografts.
Double-labeling immunofluorescence for beta-galactosidase, involucrin, and integrin beta1.
TaqMan PCR to determine genome copy number.

Main Results:

High transgene expression was observed in dermal fibroblasts transduced with VSV-G, EboZ, or MuLV pseudotyped vectors.
VSV-G, EboZ, and MuLV pseudotyped vectors effectively targeted quiescent epidermal stem cells, leading to transgene expression in differentiated progeny.
VSV-G pseudotyped vector demonstrated the highest efficiency for cutaneous transduction, indicated by superior beta-galactosidase expression and genome copy number.

Conclusions:

Pseudotyping lentiviral vectors with specific viral glycoproteins can achieve efficient gene transfer into human skin.
VSV-G is the most potent viral glycoprotein for pseudotyping HIV-based vectors for effective gene delivery to epidermal stem cells in the skin.