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Microfluidic flow control on charged phospholipid polymer interface.

Yan Xu1, Madoka Takai, Tomohiro Konno

  • 1Department of Materials Engineering, School of Engineering and Center for NanoBio Integration, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Lab on a Chip
|February 3, 2007
PubMed
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A novel charged phospholipid polymer biointerface effectively controls electroosmotic flow (EOF) and minimizes protein adsorption in microfluidic chips. This surface modification offers stable, pH-independent EOF, crucial for biomolecule compatibility in miniaturized systems.

Area of Science:

  • Surface Chemistry
  • Materials Science
  • Microfluidics

Background:

  • Controlling electroosmotic flow (EOF) and preventing non-specific protein adsorption are critical challenges in microfluidic devices for biological applications.
  • Existing surface modification methods often lack stability, pH independence, or compatibility with biomolecules.

Purpose of the Study:

  • To develop a one-step surface modification strategy for quartz microfluidic chips to control EOF and suppress protein adsorption.
  • To create a charged phospholipid polymer biointerface with tunable surface charge density for stable and predictable microchannel performance.

Main Methods:

  • Synthesis of a negatively charged phospholipid copolymer (PMBSSi) containing MPC, BMA, PMPS, and MPTMSi moieties.
  • One-step chemical bonding of the synthesized copolymer onto silica-based microchannels.

Related Experiment Videos

  • Characterization of surface properties, including zeta-potential and EOF mobility, under varying pH and copolymer concentrations.
  • Assessment of protein adsorption using model proteins (albumin and cytochrome c).

    • Main Results:

    • The PMBSSi-modified surface exhibited significant, stable cathodic EOF at neutral pH, approximately half that of the unmodified channel.
    • Surface charge density and EOF mobility were tunable by adjusting the concentration of charged (PMBSSi) and non-charged (PMSi) copolymers.
    • The modified microchannels showed minimal EOF variation across a wide pH range (1-10) and effectively suppressed non-specific adsorption of both anionic and cationic proteins.

    Conclusions:

    • The charged phospholipid polymer biointerface provides a robust and versatile method for controlling EOF in microfluidic systems.
    • This approach offers excellent protein resistance and pH stability, making it highly suitable for sensitive biological applications.
    • The developed charged interface presents a promising solution for advanced microfluidic devices requiring precise fluid control and biomolecule compatibility.