Allicin characterization and its determination by HPLC
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Published on: November 11, 2022
H Jansen1, B Müller, K Knobloch
1Institut für Botanik und Pharmazeutische Biologie der Universität Erlangen-Nürnberg, Staudtstr. 5, D-8520 Erlangen, Federal Republic of Germany.
Abstract:
Allicin has been synthesized and was purified by liquid chromatography. HPLC chromatograms recorded at different wavelengths reveal pure allicin at similar retention times. The purity of the allicin peak could be confirmed by diode array detection. Pure synthetic allicin has been identified by infrared and mass spectrometry and its spectra were characterized. For the quantitative determination of allicin from different sources, an external standard method has been elaborated.
