[Isolation and identification of a phenanthrene-degrading bacterial strain]

Through selective enriched culture, a phenanthrene-degrading bacterial strain was isolated from the oil-contaminated soil in Shenfu irrigation area of Shenyang, Northeast China. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that this strain was belonged to genus Acinetobacter and named as Acinetobacter sp. L2, and closest to Acinetobacter sp. DG880 [AY258108]. It could use phenanthrene as the sole carbon source. After 7 days culture, the degradation rate of phenanthrene was 96.3%. According to the activity of catechol 2,3-dioxygenase, the strain probably had phenanthrene-degrading genes.