Related Experiment Video

Updated: Jul 17, 2026

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
07:42

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

Proteomic analysis of tyrosine phosphorylations in vascular endothelial growth factor- and reactive oxygen

Young Mee Kim1, Eun Joo Song, Jawon Seo

  • 1The Center for Cell Signaling & Drug Discovery Research, College of Pharmacy and Division of Life & Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea.

Journal of Proteome Research
|February 3, 2007
PubMed

Insights

Vascular endothelial growth factor (VEGF) activates signaling pathways in endothelial cells. This study identified 95 differentially phosphorylated proteins, revealing distinct responses to VEGF versus hydrogen peroxide (H2O2).

Area of Science:

  • Cellular biology
  • Biochemistry
  • Molecular signaling

Background:

  • Vascular endothelial growth factor (VEGF) is crucial for angiogenesis, activating tyrosine kinase receptors.
  • VEGF signaling in endothelial cells involves reactive oxygen species (ROS) production and MAPK pathway activation.
  • Understanding VEGF receptor targets is key to elucidating angiogenic mechanisms.

Purpose of the Study:

  • To identify and semiquantify tyrosine-phosphorylated proteins regulated by VEGF signaling.
  • To compare the protein phosphorylation profiles induced by VEGF and hydrogen peroxide (H2O2).
  • To investigate differential protein phosphorylation responses within heterogeneous cell populations.

Main Methods:

  • Treatment of human umbilical vein endothelial cells (HUVECs) with VEGF or H2O2.
  • Proteomic analysis using 2D-gel electrophoresis, Western blotting with anti-phosphotyrosine antibody, and mass spectrometry.
  • Bioinformatic analysis of differentially phosphorylated proteins.

Main Results:

  • Identified 95 differentially tyrosine-phosphorylated proteins.
  • Observed proteins specifically phosphorylated by VEGF, distinct from H2O2 effects.
  • Demonstrated heterogeneous phosphorylation responses within protein subpopulations to VEGF and H2O2.

Conclusions:

  • VEGF and H2O2 induce distinct protein phosphorylation patterns in endothelial cells.
  • Differential phosphorylation suggests complex regulatory mechanisms in VEGF-mediated signaling.
  • Further bioinformatic analysis will illuminate VEGF- and H2O2-induced signaling pathways.

More Related Videos

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS07:33

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS

Published on: December 21, 2011

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors09:04

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

Published on: March 15, 2016

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...