Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

[Microwaves in morphologic examinations: technologic progress or whim?].

A Niezabitowski¹, J Stachura

¹Zakład Patologii Nowotworów Centrum Onkologii, Kraków.

Patologia Polska

|January 1, 1991

Summary

Microwave ovens significantly reduce formaldehyde exposure and processing time for microscopy tissue preparation. This method enhances staining quality and can be used for various laboratory procedures.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Decreased expression of MLH1, MSH2, PMS1 and PMS2 in pigmented lesions indicates accumulation of failed DNA repair along with malignant transformation and tumour progression.

Oncology reports·2011

Same author

Differences of ploidy status in progression of head and neck melanomas.

International journal of oncology·2011

Same author

Optimization of the method of RNA isolation from paraffin blocks to assess gene expression in breast cancer.

Polish journal of pathology : official journal of the Polish Society of Pathologists·2008

Same author

Primary adrenal tumors--a 16-year experience in a single institution.

Polish journal of pathology : official journal of the Polish Society of Pathologists·2008

Same author

Gastric secretion, proinflammatory cytokines and epidermal growth factor (EGF) in the delayed healing of lingual and gastric ulcerations by testosterone.

Inflammopharmacology·2007

Same author

Exposition of newborn rats to bacterial endotoxin impairs pancreatic enzyme secretion at adult age.

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2007

Area of Science:

Histology and Microscopy
Biotechnology
Pathology

Background:

Traditional tissue fixation for light and electron microscopy often involves lengthy procedures and hazardous chemicals like formaldehyde.
Microwave technology offers a potential alternative to accelerate and improve these critical laboratory processes.

Discussion:

Microwave irradiation effectively shortens tissue fixation times and minimizes formaldehyde concentration, enhancing laboratory safety.
The application of microwave technology extends to tissue dehydration, paraffin embedding, and staining of various specimen types.
This method shows significant improvements in immunohistochemical and ultracytochemical staining results.

Key Insights:

Microwave ovens provide a faster and safer alternative for tissue fixation in microscopy.
The technology enhances the quality of complex staining techniques, including immunohistochemistry and ultracytochemistry.
Both specialized laboratory and domestic microwave ovens can be utilized for these histological applications.

Outlook:

Further research could explore optimizing microwave parameters for specific tissue types and staining protocols.
Wider adoption of microwave-assisted techniques could lead to more efficient and safer histology laboratories.
Investigating the long-term preservation quality of microwave-treated tissues is warranted.

Related Experiment Videos