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Updated: Jul 17, 2026
08:48
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
A BAC-based physical map of the apple genome
Yuepeng Han1, Ksenija Gasic, Brandy Marron
1Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL 61801, USA.
Genomics
|February 3, 2007
Summary
Scientists created a physical map of the apple genome using BAC clones. This genome map is the first for any Rosaceae family member or tree species, aiding future genomics research.
Area of Science:
- Genomics
- Plant Science
- Bioinformatics
Background:
- Genome-wide physical mapping is crucial for understanding complex traits and advancing genomics in various species.
- A comprehensive physical map is lacking for many important plant groups, including tree species.
Purpose of the Study:
- To construct a high-resolution, genome-wide physical map of the apple (Malus domestica) genome.
- To provide a foundational resource for advanced genomics research in apple and other tree species.
Main Methods:
- Utilized 74,281 bacterial artificial chromosome (BAC) clones, representing approximately 10.5x haploid genome equivalents.
- Assembled BAC fingerprints into contigs using variable stringencies and individual library data.
- Validated contig assembly through consensus mapping and DNA marker analysis.
Main Results:
- Successfully constructed a physical map comprising 2702 contigs, spanning an estimated 927 Mb.
- Demonstrated high reliability of contig assembly through rigorous evaluation methods.
- This BAC-based physical map is the first for the Rosaceae family and all tree species.
Conclusions:
- The developed apple genome physical map is a reliable resource for future genomic studies.
- Facilitates marker development, gene isolation, comparative genomics, and large-scale sequencing in apple and other tree species.
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