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High-Throughput Analysis of Optical Mapping Data Using ElectroMap

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Simulations of optical mapping during electroporation.

Deborah L Janks¹, Bradley J Roth

¹Department of Physics, Oakland University, Rochester, Michigan, USA.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference

|February 3, 2007

Summary

Optical mapping underestimates cardiac electroporation thresholds by averaging transmembrane potential (Vm) over depth. Numerical simulations confirm this underestimation, suggesting Vm exceeds +/- 400 mV at the tissue surface.

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Area of Science:

Biophysics
Cardiac Electrophysiology
Computational Biology

Background:

Electroporation in cardiac tissue is experimentally linked to transmembrane potential (Vm) below +/- 400 mV.
Optical mapping techniques may underestimate Vm due to spatial averaging over tissue depth.

Purpose of the Study:

To test the hypothesis that optical mapping underestimates Vm during cardiac electroporation.
To investigate the spatial distribution of Vm at the tissue surface versus depth.
To explore the underestimation of resting transmembrane potential (Vrest) depolarization.

Main Methods:

Numerical simulations of cardiac tissue electrophysiology.
Modeling of transmembrane potential (Vm) under electrical stimulation.
Comparison of surface Vm with depth-averaged Vm.

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Main Results:

Numerical simulations confirmed significant underestimation of Vm by optical mapping.
Electroporation was predicted to occur only near the tissue surface in a homogeneous model.
A similar underestimation of Vrest depolarization is hypothesized.

Conclusions:

Optical mapping may overestimate electroporation thresholds in cardiac tissue.
Depth-dependent Vm averaging is a critical factor in interpreting optical mapping data.
Further investigation into Vrest depolarization underestimation is warranted.