Deletion/insertion polymorphism of the prion protein gene (PRNP) in Polish Holstein-Friesian cattle
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Updated: Jul 17, 2026

Detection of Abnormal Prion Protein by Immunohistochemistry
Published on: May 5, 2023
Urszula Czarnik1, Tadeusz Zabolewicz, Janusz Strychalski
1Department of Animal Genetics, University of Warmia and Mazury, M. Oczapowskiego 5, 10-957 Olsztyn, Poland. czar@uwm.edu.pl
Abstract:
The aim of the present study was to identify the deletion/insertion polymorphism of the bovine prion protein gene (PRNP) within the promoter sequence (23 bp), intron 1 (12 bp) and 3' untranslated region (14 bp). DNA was isolated from blood of 234 randomly tested Polish Holstein-Friesian cows and from semen of 47 sires used for artificial insemination (AI) in 2004. No statistically significant differences were found in the frequency of genotypes and alleles between cows and breeding bulls in the 3 analysed polymorphic sites within the PRNP gene. Only 3 haplotypes were identified in sires and 4 haplotypes in cows.
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