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Ultrastructure and virus particles of L1210/Mes. A hamster-adapted murine leukemia
Abstract:
The ultrastructural features and virus particle content of L1210/Mes., the hamster-adapted strain of murine leukemia L1210, has been studied in cheek pouch tumors and cell cultures. In comparison with the parental murine tumor in culture, L1210/Mes. has experienced certain morphologic alterations, such as a striking increase in cell size, enlarged and more bizarre nuclei, and an increase in the number of lysosome-related structures. Type A (intracytoplasmic and intracisternal) and type C virus particles could be demonstrated in cell cultures of murine leukemia L1210, and in cheek pouch grafts and cell cultures of L1210/Mes. No. "H" (or "R") hamster-associated virus particles, however, could be distinguished in L1210/Mes. tumors or in vitro cultivated cells. It appears that a murine lymphoma capable of serial propagation in a xenogeneic host permits the replication of virus particles structurally indistinguishable from those indigenous to it.
Insights
Hamster-adapted murine leukemia L1210/Mes. showed morphologic changes and retained its native murine leukemia virus particles. Xenogeneic host adaptation did not introduce hamster-associated viruses.
Area of Science:
- Oncology
- Virology
- Cell Biology
Background:
- Murine leukemia L1210 is a well-characterized lymphoid tumor.
- Adaptation of tumors to xenogeneic hosts can alter their biological and virological properties.
Purpose of the Study:
- To investigate the ultrastructural characteristics and virus particle content of L1210/Mes., a hamster-adapted strain of murine leukemia L1210.
- To compare the viral profile of the adapted strain with the parental murine tumor.
Main Methods:
- Ultrastructural analysis using electron microscopy.
- Examination of cheek pouch tumors and cell cultures.
- Comparison of L1210/Mes. with the parental murine tumor in vitro.
Main Results:
- L1210/Mes. exhibited significant morphologic alterations compared to the parental strain, including increased cell size and nuclear irregularities.
- Type A and Type C murine leukemia virus particles were detected in both parental and adapted L1210/Mes. cells.
- No hamster-associated viruses (Type "H" or "R") were identified in the L1210/Mes. tumors or cell cultures.
Conclusions:
- Serial propagation of a murine lymphoma in a xenogeneic host (hamster) allows for the replication of its indigenous murine virus particles.
- Xenogeneic adaptation of L1210 leukemia does not lead to the acquisition or expression of hamster-associated viruses.