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Related Experiment Video

Updated: Jul 17, 2026

Engineered Vascularized Muscle Flap
08:18

Engineered Vascularized Muscle Flap

Published on: January 11, 2016

Tissue engineered prefabricated vascularized flaps.

Kenneth Kian Kwan Oo1, Wei Chen Ong, Annette Hui Chi Ang

  • 1Department of Otolaryngology, Head and Neck Surgery, National University of Singapore, Singapore, Singapore. kkkwco@yahoo.com

Head & Neck
|February 3, 2007
PubMed
Summary

Tissue engineering combined with flap prefabrication created a vascularized soft tissue flap using human dermal fibroblasts on a polylactic-co-glycolic acid-collagen scaffold. This approach successfully generated viable, vascularized tissue for potential reconstruction applications.

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Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Vascular Biology

Background:

  • Microvascular free tissue transfer is common for head and neck reconstruction but has drawbacks.
  • Tissue engineering offers neo-tissue but often lacks vascularization.
  • This study explores combining tissue engineering with flap prefabrication for vascularized flaps.

Purpose of the Study:

  • To develop a prefabricated vascularized soft tissue flap using tissue engineering techniques.
  • To assess the viability and vascularization of engineered tissue constructs.

Main Methods:

  • Human dermal fibroblasts (HDFs) were seeded onto polylactic-co-glycolic acid-collagen (PLGA-c) mesh.
  • Constructs were implanted onto a ligated rat femoral artery and vein for 4 weeks.

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Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
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Last Updated: Jul 17, 2026

Engineered Vascularized Muscle Flap
08:18

Engineered Vascularized Muscle Flap

Published on: January 11, 2016

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
08:31

Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies

Published on: May 19, 2022

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
10:56

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

  • Assessment included morphology, histology, and cell viability via fluorescence.

    • Main Results:

    • Constructs maintained shape with evident angiogenesis and neo-capillary formation.
    • Seeded HDFs proliferated, filling the scaffold and remaining viable.
    • Increased collagen formation was observed in HDF-seeded constructs compared to controls.

    Conclusions:

    • Polylactic-co-glycolic acid-collagen (PLGA-c) mesh with fibroblasts forms a suitable scaffold for soft tissue flaps.
    • A ligated arteriovenous pedicle can serve as a vascular carrier for engineered vascularized flaps.