Processing methods of ultrathin poly(epsilon-caprolactone) films for tissue engineering applications
Kay Siang Tiaw1, Swee Hin Teoh, Ran Chen
1Centre for Biomedical Materials Applications and Technology (BIOMAT), Department of Mechanical Engineering, National University of Singapore, 10 Kent Ridge Crescent, 119260 Singapore.
Biomacromolecules
|February 6, 2007
Summary
Ultrathin poly(epsilon-caprolactone) (PCL) films fabricated via different methods exhibit varying properties. Spin casting yields the thinnest films, while biaxial drawing enhances mechanical strength for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Polymer Science
- Tissue Engineering
Background:
- Poly(epsilon-caprolactone) (PCL) is a versatile biodegradable polymer.
- Fabrication methods significantly influence PCL film properties.
- Developing ultrathin PCL films is crucial for advanced biomedical applications.
Purpose of the Study:
- To fabricate and characterize ultrathin PCL films using spin casting, 2-roll milling, and solution casting.
- To investigate the impact of biaxial drawing on film morphology, thermal properties, and mechanical strength.
- To correlate fabrication techniques with water vapor transmission rate (WVTR) for potential tissue engineering uses.
Main Methods:
- Fabrication of PCL films via spin casting, 2-roll milling, and solution casting.
- Biaxial drawing to achieve ultrathin film dimensions.
- Morphological analysis using polarized microscopy and atomic force microscopy (AFM).
- Thermal analysis using differential scanning calorimetry (DSC).
- Measurement of water vapor transmission rate (WVTR).
- Tensile testing for mechanical characterization.
Main Results:
- Spin cast films achieved the thinnest (1.2 µm) after biaxial drawing.
- 2-roll mill films showed the highest drawing ratio (4x4), while spin cast films reached 2x2.
- Film morphology varied, with spin cast films exhibiting finer fibrillar networks.
- Biaxial drawing increased peak-melting temperature and decreased crystallinity.
- WVTR was dependent on fabrication method and inversely proportional to film thickness.
- Tensile strength and modulus significantly improved after biaxial stretching.
Conclusions:
- Fabrication technique critically determines PCL film morphology, thickness, and properties.
- Biaxially drawn ultrathin PCL films offer enhanced mechanical performance.
- The distinct properties of PCL films produced by different methods allow for tailored applications in tissue engineering.
- Potential applications include dermatology, ophthalmology, vascular grafts, and soft tissue regeneration.
