Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

Amperometry: Overview

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

DUNI: A Portable Smartphone-Coupled Integrating Sphere for Controlled Illumination and Reliable Colorimetric Sensing: Analytical Applications.

Sensors (Basel, Switzerland)·2026

Same author

Kinetic and analytical characterization of a new tropinone oxidase enzyme and its application to the simultaneous determination of the tropane alkaloids atropine and scopolamine.

Analytical and bioanalytical chemistry·2025

Same author

In situ Metal-Enhanced Fluorescence of gold nanoclusters for enzymatic biosensors.

Talanta·2025

Same author

NADPH-mediated seedless in situ formation of gold or gold-platinum nanoparticles for the enzymatic determination of atropine.

Mikrochimica acta·2025

Same author

Towards new fluorometric methodologies based on the in-situ generation of gold nanoclusters.

Talanta·2023

Same author

Tectomer-Mediated Optical Nanosensors for Tyramine Determination.

Sensors (Basel, Switzerland)·2023

Search research articles

Related Experiment Video

Updated: Jul 17, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Direct glucose determination in blood using a reagentless optical biosensor.

Vanesa Sanz¹, Susana de Marcos, Javier Galbán

¹ABG (Analytical Biosensors Group), INA (Instituto de Nanociencia de Aragón), Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, 50009 Zaragoza, Spain.

Biosensors & Bioelectronics

|February 6, 2007

Summary

This study presents a novel reagentless biosensor for direct blood glucose determination using intrinsic spectroscopic properties. This method simplifies analysis by eliminating sample pretreatment, offering a stable and efficient diagnostic tool.

More Related Videos

Bergmeyer Glucose Quantification for Microbiological Samples

Bergmeyer Glucose Quantification for Microbiological Samples

Published on: January 17, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Related Experiment Videos

Last Updated: Jul 17, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

Bergmeyer Glucose Quantification for Microbiological Samples

Bergmeyer Glucose Quantification for Microbiological Samples

Published on: January 17, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biosensor Technology

Background:

Accurate glucose monitoring is crucial for diabetes management.
Existing methods often require complex sample pretreatment.
Need for rapid, reagentless, and direct blood glucose measurement techniques.

Purpose of the Study:

To develop and demonstrate a reagentless biosensor for direct blood glucose determination.
To utilize the intrinsic spectroscopic properties of enzymes for sensing.
To establish a simplified and efficient method for blood glucose analysis.

Main Methods:

Immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOx) in a polyacrylamide gel matrix.
Development of a reagentless biosensor utilizing intrinsic spectroscopic properties.
Direct analysis of diluted blood samples without pretreatment, with absorption correction.

Main Results:

The biosensor operates in continuous mode with a linear response range from 1.5 x 10(-6) to 5.5 x 10(-5) M.
Achieved a minimum sample rate of 30 samples per hour.
Demonstrated stability for at least 750 measurements.
Successfully modeled biosensor signals for potential application to other compounds.

Conclusions:

A reagentless biosensor enables direct and efficient blood glucose determination.
The developed method simplifies blood glucose analysis by eliminating pretreatment steps.
The biosensor technology shows promise for broader applications in biochemical compound detection.