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Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

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Related Experiment Video

Updated: Jul 17, 2026

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients

Published on: May 24, 2019

Novel cell culture device enabling three-dimensional cell growth and improved cell function.

Maria Bokhari¹, Ross J Carnachan, Neil R Cameron

¹School of Biological and Biomedical Science, Durham University, South Road, Durham, UK.

Biochemical and Biophysical Research Communications

|February 6, 2007

Summary

This study introduces a new 3-D cell culture device using porous polystyrene scaffolds. This technology enhances liver cell function compared to traditional 2-D cultures, advancing in vitro cell biology research.

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Area of Science:

Biotechnology
Cell Biology
Tissue Engineering

Background:

Understanding cell biology and cell-cell interactions necessitates advanced in vitro models.
Existing 2-D culture systems do not fully replicate the in vivo tissue microenvironment.
Three-dimensional (3-D) culture systems offer a more physiologically relevant approach.

Purpose of the Study:

To present a novel device for routine 3-D cell growth in vitro.
To develop and adapt a porous polystyrene scaffold for cell culture applications.
To evaluate the performance of liver cells cultured in the 3-D system.

Main Methods:

Development of a thin membrane of polystyrene with a uniform porous architecture.
Culturing HepG2 liver cells on both 2-D and 3-D porous polystyrene substrates.
Comparative analysis of HepG2 cell functional activity between 2-D and 3-D cultures.

Main Results:

HepG2 cells grown on the 3-D porous polystyrene scaffolds exhibited significantly enhanced functional activity.
The novel device successfully facilitated routine 3-D cell growth.
The porous polystyrene scaffold demonstrated suitability for cell culture applications.

Conclusions:

The developed device represents a new technology for routine 3-D cell culture.
Porous polystyrene scaffolds improve the performance of cultured liver cells compared to 2-D cultures.
This technology advances in vitro modeling for cell biology and tissue engineering.