Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Trends in sample preparation for classical and second generation proteomics.

Benito Cañas1, Carmen Piñeiro, Enrique Calvo

  • 1Dept. Química Analítica, Facultad de CC, Químicas, UCM, Av.Complutense s/n, Madrid 28040, Spain. bcanasmo@quim.ucm.es

Journal of Chromatography. A
|February 6, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Grape Seed Proanthocyanidins Enhance Time-Dependent HO-1 Activation and Improve Redox Homeostasis in Obesity-Induced Hepatic Dysfunction.

Antioxidants (Basel, Switzerland)·2026
Same author

OMA1 protects from liver injury and tumorigenesis during aging by controlling hepatic immunogenicity.

The EMBO journal·2026
Same author

Mitochondria directly interact with the nuclear pore complex.

Nature·2026
Same author

Pro-regenerative fingerprints identified in a sub-population of adult mouse cardiomyocytes by integrative single-cell proteomics.

Genome biology·2026
Same author

Adiponectin modulates the diurnal hepatic transcriptome and energy metabolism in male mice.

Endocrine connections·2026
Same author

Mitochondrial remodeling in skeletal muscle underlies exercise-induced reversal of age-associated functional decline in mice and humans.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
See all related articles

This review compiles essential protein sample preparation strategies for proteomics. It details methods for protein extraction, identification, and quantification using classical and advanced techniques.

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Sample preparation is a critical, yet often underexplored, component of proteomics workflows.
  • A comprehensive update on protein extraction and identification strategies is needed for researchers.

Purpose of the Study:

  • To review and compile current strategies and protocols for protein sample preparation in proteomics.
  • To cover both classical and second-generation proteomics methodologies.

Main Methods:

  • Review of established and novel techniques for tissue disruption and cell lysis.
  • Description of protein pre-fractionation and separation methods, including 2-Dimensional Electrophoresis (2-DE).
  • Overview of protein digestion, mass spectrometry analysis, and multidimensional peptide separations for quantification.

Related Experiment Videos

Main Results:

  • Detailed description of various procedures for each step of the proteomics workflow.
  • Inclusion of methods for quantifying protein expression levels.

Conclusions:

  • This review provides a centralized resource for protein sample preparation techniques.
  • It aims to guide researchers in optimizing their proteomics experiments for accurate protein identification and quantification.