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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Reversed-phase liquid chromatography column testing and classification: physicochemical interpretation based on a
K Le Mapihan1, J Vial, A Jardy
1Laboratoire "Environnement et Chimie Analytique", UMR 7121, Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris 10, rue Vauquelin, 75005 Paris, France. katell.lemapihan@espci.fr
Abstract:
The high number of stationary phases commercially available for liquid chromatography makes the choice of the analyst a real headache. In order to provide a tool to carry out this choice on objective basis, the present work proposes interpretations of the column classifications obtained, thanks to a previously described testing procedure. The meaning of principal components was attributed to crossing over information carried by loading plots and groups revealed by hierarchical cluster analysis (HCA) on the corresponding score plots. At high solvent ratio, the retention seemed to be governed by enthalpy, whereas at low solvent ratio, entropic phenomena were predominating. Finally, the behavior of known families of RPLC columns was studied giving rise either to homogeneous groups like polar embedded grafts columns or to scattered families like Aqua type columns.
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