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Updated: Jul 17, 2026
06:40
Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
Published on: November 4, 2018
A host cell membrane protein, golgin-97, is essential for poxvirus morphogenesis
Dina Alzhanova1, Dennis E Hruby
1Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331, USA.
Virology
|February 6, 2007
Summary
Golgin-97, a cellular protein, is crucial for enveloped virus replication. Its depletion prevents the formation of mature infectious viruses, highlighting its role in virus morphogenesis and maturation.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Enveloped virus replication involves critical steps like membrane acquisition and genome encapsidation.
- The biogenesis and maturation mechanisms of poxviral primary membranes and cores remain largely unknown.
- Understanding these processes is key to controlling viral infections.
Purpose of the Study:
- To investigate the role of cellular proteins in poxvirus replication and morphogenesis.
- To identify specific cellular factors essential for the maturation of infectious poxviruses.
Main Methods:
- Utilized an RNA interference (RNAi) approach to deplete specific cellular proteins.
- Analyzed virion morphology in cells lacking the target protein using microscopy.
- Compared the formation of mature and immature virus forms.
Main Results:
- Demonstrated that the cellular trans-Golgi network protein, golgin-97, is essential for virus replication.
- Cells depleted of golgin-97 exhibited defects in virion morphogenesis.
- Specifically, golgin-97 is required for the formation of mature infectious virus, not immature precursors.
Conclusions:
- Golgin-97 plays a vital role in the maturation of poxviruses.
- The protein is likely involved in the maturation of the virus core and/or its outer membrane.
- Further research into golgin-97's function can reveal new antiviral strategies.
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