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In vivo demonstration of FNR dimers in response to lower O(2) availability
Adrian J Jervis1, Jeffrey Green
1Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom.
Abstract:
Escherichia coli FNR is an O(2)-sensing transcription factor. In vitro studies indicate that anaerobic iron-sulfur cluster acquisition promotes FNR dimerization. Here, two-hybrid assays show that iron-sulfur cluster-dependent FNR dimers are formed in vivo in response to lower O(2) availability, consistent with the current model of FNR activation.
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