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Updated: Jul 17, 2026
Microarray Analysis for Saccharomyces cerevisiae
Published on: April 7, 2011
Purification, crystallization and preliminary X-ray analysis of Hsp33 from Saccharomyces cerevisiae
Wei Liu1, Ye-Yun Zhou, Mai-Kun Teng
1Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
Abstract:
The heat-shock protein Hsp33 from the yeast Saccharomyces cerevisiae has been overexpressed, purified and crystallized. A crystal was obtained using the hanging-drop vapour-diffusion method and a data set was collected to 2.7 A resolution. The crystal belongs to space group P4(3)2(1)2, with unit-cell parameters a = b = 96.43, c = 132.22 A, alpha = beta = gamma = 90 degrees . The asymmetric unit is assumed to contain two subunits of Hsp33, with a V(M) value of 2.96 A(3) Da(-1) and a solvent content of 58.41%.
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