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Related Experiment Video

Updated: Jul 17, 2026

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine

Establishment of Coloproctitis Cancer Model in Mice and Evaluation of Therapeutic Effect of Chinese Medicine

Published on: October 13, 2023

[Conventional histological diagnostics in coloproctology].

W A Meier-Ruge¹, E Bruder

¹Institut für Pathologie der Universität Basel, Schönbeinstrasse 40, 4031 Basel, Switzerland. meier-ruge@bluewin.ch

|February 7, 2007

Summary

Immunohistochemistry aids coloproctology diagnosis, with specific stains identifying hypoganglionosis and desmosis. Enzyme histochemistry remains crucial for ultrashort Hirschsprung

Area of Science:

Gastroenterology
Histopathology
Coloproctology

Context:

Histopathological diagnosis in coloproctology has advanced with immunohistochemical (IHC) methods.
Formalin-fixed, paraffin-embedded tissues are standard for these analyses.

Purpose:

To review the utility of IHC and enzyme histochemistry in diagnosing coloproctological conditions.
To highlight the complementary roles of various histopathological techniques.

Summary:

IHC stains like S100, cathepsin D, and picrosirius red are effective for diagnosing hypoganglionosis and desmosis.
CD 117 IHC aids in evaluating Cajal cells.
Acetylcholinesterase (AChE) enzyme histochemistry is indispensable for diagnosing ultrashort Hirschsprung's disease, as IHC cannot replace it.

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Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

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Enzyme histochemistry requires native cryostat sections, necessitating organized biopsy handling.

Impact:

Improved diagnostic accuracy for specific myenteric plexus and muscularis propria disorders.
Defines the essential role of enzyme histochemistry for certain congenital conditions.
Emphasizes the need for optimized biopsy procedures for enzyme histochemistry.